Microscopy
The division has a Zeiss Axioplan Universal microscope equipped with differential interference contrast and a High Resolution Microscopy Camera AxioCam MRm Rev. 3 FireWire, Illuminator HBO 100 as well as with a 100W mercury short-arc lamp and a system of filters to allow the fluorescence microscopy observations. The microscope is further equipped with a high-sensitivity SIT video camera and an image processor, AxioVision 4 together with the Macintosh-based image analysis software.
Sponsored by FRN & Crafoord Foundation.
Contact
Contact person: Emma Sparr
This instrument allows recording brilliant, high-resolution images to illustrate morphological features of fixed or slowly moving samples as well as monitoring high-speed dynamic processes by fast time-course studies. The equipment is implemented with five true spectral confocal channels simultaneously with a prism spectrometer for high transmittance and tunability. Illumination regimes are switchable in microseconds for fast dynamic measurement and the beam can be split instantly for new dyes or laser lines. The apparatus mounts up to 2 channels for spectral FLIM allowing resolved fluorescence life-time imaging and 3 laser lines: a HeNe laser (543 and 633 nm), an Argon laser (458, 476, 488 and 514 nm) and an IR (800 to 1100 nm). A fast resonant scanner (50 frames/sec at 512 x 256 pixels) and a non-resonant scanner (1400 lines/sec) are also implemented. The objective is mounted on a piezo-stage for fast z-scanning (50 frames/sec at 256 x 128 pixels).
In 2012, the system was upgraded with SMD Detection package FCS (high quantum efficiency, 2 APD). The system acquires and analyzes FCS and FCCS (Fluorescence Cross-Correlation Spectroscopy) data. Both methods focus on quantitative analysis of transport and binding processes.
Sponsored by The Knut and Alice Wallenberg Foundation.
Contact
Contact person: Peter Holmqvist
The division has a custom made setup for high-resolution fluorescence imaging capable of single molecule studies, fluorescence recovery after photobleaching (FRAP) and total internal reflection fluorescence (TIRF) imaging at two wavelengths simultaneously (488 nm and 638 nm excitation). The setup is also combined with a piezo-controlled (with nm resolution) holder for nano- and micropipette manipulation and delivery. The setup is based around a Nikon Eclipse TE2000 U microscope, with a Nikon Apo TIRF 60x (NA = 1.49) oil objective for the TIRF studies. Other objectives available are: 10x, 20x, and 60x air objectives. Excitation light comes from two Cobolt MLD diode lasers operating at 488 nm (maximum 60 mW power) and 638 nm (maximum 140 mW power). The excitation laser power is adjusted with a motorized filter wheel from Thorlabs. Images are collected with a sCMOS camera from Hamamatsu (ORCA-Flash4.0 LT). The software used to control the setup is the open source program Micro-Manager.
Funding from the Royal Physiographic Society in Lund, the Royal Swedish Society, Per-Eric and Ulla Schyberg’s Foundation, and the Crafoord Foundation is gratefully acknowledged.
Contact
Contact person: Peter Jönsson
The division has a custom-made fluorescence microscope capable of super-resolution imaging (STORM/PALM) simultaneously at two wavelengths. The setup is based around a Nikon Eclipse Ti-E microscope, with a Nikon Apo TIRF 100x (NA = 1.49) oil objective. Excitation light comes from either of four Oxxius diode lasers operating at 405 nm (100 mW power), 488 nm (100 mW power), 561 (100 mW power) and 638 nm (180 mW power). Images are collected with a sCMOS camera from Photometrics (Prime 95B). The software used to control the setup is the open source program Micro-Manager.
Funding from the Knut and Alice Wallenberg Foundation and the Carl Tesdorpf Foundation is gratefully acknowledged.
Contact
Contact person: Peter Jönsson