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Ulf Olsson. Portrait.

Ulf Olsson

Professor

Ulf Olsson. Portrait.

Temperature Induced DNA Compaction in a Nonionic Lamellar Phase

Author

  • Alfredo Gonzalez Perez
  • Sanja Bulut
  • Ulf Olsson
  • Björn Lindman

Summary, in English

A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.

Department/s

  • Physical Chemistry

Publishing year

2008

Language

English

Pages

174-180

Publication/Series

Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)

Volume

135

Document type

Paper in conference proceeding

Publisher

Springer

Topic

  • Physical Chemistry (including Surface- and Colloid Chemistry)

Keywords

  • compaction
  • surfactant
  • DNA
  • decompaction
  • Mesh phase
  • Nonionic
  • C10E3
  • lamellar phase

Conference name

9th International Conference on Colloid Chemistry, 1997

Conference date

1997-07-06 - 1997-07-12

Conference place

Siofok, Hungary

Status

Published

Report number

135

ISBN/ISSN/Other

  • ISBN: 978-3-540-85133-2
  • ISSN: 0340-255X