From: 2024-08-27 14:15 to 15:00
Place: Lecturehall KC:F
Contact: felix [dot] roosen-runge [at] fkem1 [dot] lu [dot] se

A Physical Chemistry seminar by Docent Stefan Wennmalm, SciLifeLab, Royal Institute of Technology-KTH, Stockholm.

Title: FCS and super-resolution techniques available at Scilifelab in Stockholm

Abstract

The Advanced Light Microscopy facility (ALM) at Scilifelab Stockholm hosts microscopes for Fluorescence Correlation Spectroscopy (FCS) and super-resolution in the form of STED- and PALM/STORM-imaging as well as MINFLUX imaging and tracking. I will in this talk introduce these techniques and give a few examples of how they have been of help to different research groups around Sweden.

FCS is an established technique for analyzing molecular interactions in solution and in living cells. The diffusion of fluorescently labeled biomolecules gives information about concentrations, diffusion coefficients, molecular sizes and interactions, and even rotational motion and conformational fluctuations. Measurements are typically performed at nanomolar concentrations.

STED- and PALM/STORM-imaging are the first-generation super-resolution techniques that generate fluorescence images with a resolution of about 30 nm which is ten times better than standard confocal microscopes.

MINFLUX is the second-generation super-resolution technique which generates images with about 5 nm resolution. MINFLUX can also track single biomolecules in living cells with a time-resolution of 50 microseconds.